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Coding
Salty_PduT

Part:BBa_K562006:Design

Designed by: Frank Sargent   Group: iGEM11_Dundee   (2011-09-16)


Salty_PduTU BMC Components


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 765
    Illegal BamHI site found at 1042
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 308
    Illegal AgeI site found at 293
    Illegal AgeI site found at 907
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Therewas one native PstI sites within the pduU gene. This was removed using PCR-based site-directed mutagenesis. Care was taken not to alter the primary sequence of the gene product. The fragment also carries an engineered BamHI site at its extreme 3' end.


Source

Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.

References